Nano-technology has changed the properties of metal elements’ delivery into and effect on living systems. The current study, first, evaluated different combinations of plant growth regulators NAA, 2,4-d and KIN on Nicotiana tabacum callus induction from root, internode, petiole and leaf explants. Two mg L−1 NAA with 0.1 mg L−1 KIN on Murashige and Skoog (MS) medium induced calli in all
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